Mouse Aggrecan ELISA Kit | AGC elisa kit

Mouse Aggrecan ELISA Kit

Cat. Num. AAA16069

• Gene Names: Acan; Agc; Agc1
• Reactivity: Mouse
• Synonyms: Aggrecan; N/A; Mouse Aggrecan ELISA Kit; AGC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for AGC elisa kit

Principle of the Assay: AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.

Intended Uses: This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for AGC elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 11990616
• NCBI GeneID: 58968
• NCBI Accession #: NP_071526.1
• NCBI GenBank Nucleotide #: NM_022190.1
• Molecular Weight: 225,171 Da
• NCBI Official Full Name: aggrecan core protein
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: Acan
• NCBI Official Synonym Symbols: Agc; Agc1
• NCBI Protein Information: aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage
• UniProt Protein Name: Aggrecan core protein
• UniProt Gene Name: Acan
• UniProt Synonym Gene Names: Agc; Agc1; CSPCP
• UniProt Entry Name: PGCA_RAT