Standard Curve (Sample)
Human Fetuin A ELISA Kit | AHSG elisa kit
Human Fetuin A PicoKine ELISA Kit
Gene Names
AHSG; AHS; A2HS; HSGA; FETUA
Reactivity
Human
Synonyms
Fetuin A; N/A; Human Fetuin A PicoKine ELISA Kit; Alpha-2-HS-glycoprotein; alpha-2-HS-glycoprotein; A2HS; AHS; Ahsg; Alpha 2 HS glycoprotein; Alpha-2-HS-glycoprotein chain B; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUA; FETUA_HUMAN; Fetuin-A; HSGA; AHSG elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human AHSG
Sequence Length
367
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AHSG elisa kit
Principle of the Assay: The Picokine™ Human AHSG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human AHSG with a 96-well strip plate that is pre-coated with antibody specific for AHSG. The detection antibody is a biotinylated antibody specific for AHSG. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human AHSG with immunogen: Expression system for standard: NS0; Immunogen sequence: A19-V367. The kit is analytically validated with ready-to-use reagents. To measure Human AHSG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human AHSG in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human AHSG in the sample.
Background/Introduction: Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HSglycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2
Background/Introduction: Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HSglycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,325 Da
NCBI Official Full Name
Alpha-2-HS-glycoprotein
NCBI Official Synonym Full Names
alpha-2-HS-glycoprotein
NCBI Official Symbol
AHSG
NCBI Official Synonym Symbols
AHS; A2HS; HSGA; FETUA
NCBI Protein Information
alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein
UniProt Protein Name
Alpha-2-HS-glycoprotein
UniProt Gene Name
AHSG
UniProt Synonym Gene Names
FETUA
UniProt Entry Name
FETUA_HUMAN
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Product Notes
The Human AHSG ahsg (Catalog #AAA45303) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45303 ELISA Kit recognizes Human AHSG. It is sometimes possible for the material contained within the vial of "Fetuin A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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