Standard Curve (Sample)
Mouse A Kinase Anchor Protein 1 ELISA Kit | AKAP1 elisa kit
Mouse A Kinase Anchor Protein 1 ELISA Kit
Gene Names
AKAP1; AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84
Reactivity
Mouse
Synonyms
A Kinase Anchor Protein 1; N/A; Mouse A Kinase Anchor Protein 1 ELISA Kit; AKAP1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This AKAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AKAP1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AKAP1 elisa kit
Principle of the assay: AKAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKAP1 antibody and an AKAP1-HRP conjugate. The assay sample and buffer are incubated together with AKAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP1 concentration since AKAP1 from samples and AKAP1-HRP conjugate compete for the anti-AKAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP1 from the sample, fewer sites are left to bind AKAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AKAP1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,921 Da
NCBI Official Full Name
A-kinase anchor protein 1, mitochondrial
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 1
NCBI Official Symbol
AKAP1
NCBI Official Synonym Symbols
AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84
NCBI Protein Information
A-kinase anchor protein 1, mitochondrial; AKAP 149; D-AKAP-1; protein kinase A1; tudor domain containing 17; A-kinase anchor protein 149 kDa; protein kinase A anchoring protein 1; spermatid A-kinase anchor protein 84; protein phosphatase 1, regulatory subunit 43; dual-specificity A-kinase anchoring protein 1
UniProt Protein Name
A-kinase anchor protein 1, mitochondrial
UniProt Gene Name
AKAP1
UniProt Synonym Gene Names
AKAP149; PRKA1; AKAP 149; D-AKAP-1; PRKA1; S-AKAP84
UniProt Entry Name
AKAP1_HUMAN
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Product Notes
The Mouse AKAP1 akap1 (Catalog #AAA82131) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA82131 ELISA Kit recognizes Mouse AKAP1. It is sometimes possible for the material contained within the vial of "A Kinase Anchor Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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