Standard Curve (Sample)
Monkey A kinase (PRKA) anchor protein (gravin) 12 ELISA Kit | AKAP12 elisa kit
Monkey A kinase (PRKA) anchor protein (gravin) 12 ELISA Kit
Gene Names
AKAP12; SSeCKS; AKAP250
Reactivity
Monkey
Synonyms
A kinase (PRKA) anchor protein (gravin) 12; N/A; Monkey A kinase (PRKA) anchor protein (gravin) 12 ELISA Kit; AKAP12 elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This AKAP12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AKAP12. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This AKAP12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AKAP12. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AKAP12 elisa kit
Principle of the Assay: AKAP12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKAP12 antibody and an AKAP12-HRP conjugate. The assay sample and buffer are incubated together with AKAP12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP12 concentration since AKAP12 from samples and AKAP12-HRP conjugate compete for the anti-AKAP12 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP12 from the sample, fewer sites are left to bind AKAP12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP12 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AKAP12 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
180,990 Da
NCBI Official Full Name
A-kinase anchor protein 12 isoform 2
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 12
NCBI Official Symbol
AKAP12
NCBI Official Synonym Symbols
SSeCKS; AKAP250
NCBI Protein Information
A-kinase anchor protein 12; AKAP 250; kinase scaffold protein gravin; A-kinase anchor protein, 250kDa; Src-Suppressed C Kinase Substrate; myasthenia gravis autoantigen gravin
UniProt Protein Name
A-kinase anchor protein 12
UniProt Gene Name
AKAP12
UniProt Synonym Gene Names
AKAP250; AKAP-12; AKAP 250
UniProt Entry Name
AKA12_HUMAN
Similar Products
Product Notes
The Monkey AKAP12 akap12 (Catalog #AAA96358) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA96358 ELISA Kit recognizes Monkey AKAP12. It is sometimes possible for the material contained within the vial of "A kinase (PRKA) anchor protein (gravin) 12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.