SDS-PAGE
AKR1D1 recombinant protein
AKR1D1, 1-326aa, Human, E Coli
Gene Names
AKR1D1; CBAS2; SRD5B1; 3o5bred
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
AKR1D1; N/A; AKR1D1, 1-326aa, Human, E Coli; 3o5bred; CBAS2; SRD5B1; AKR1D1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
Sequence Length
326
Applicable Applications for AKR1D1 recombinant protein
SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for AKR1D1 recombinant protein
AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AKR1D1 recombinant protein
References
Moore S A., et al. (1995) J Mol Biol. 249 (1):195-214; Gould S J., et al. (1988) Anal Biochem. 175 (1):5-13
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37.3 kDa (326aa)
NCBI Official Full Name
3-oxo-5-beta-steroid 4-dehydrogenase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member D1
NCBI Official Symbol
AKR1D1
NCBI Official Synonym Symbols
CBAS2; SRD5B1; 3o5bred
NCBI Protein Information
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Protein Name
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Gene Name
AKR1D1
UniProt Synonym Gene Names
SRD5B1
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Product Notes
The AKR1D1 akr1d1 (Catalog #AAA48919) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AKR1D1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the AKR1D1 akr1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY. It is sometimes possible for the material contained within the vial of "AKR1D1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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