Standard Curve (Sample)
Mouse CD166 ELISA Kit | ALCAM elisa kit
Mouse CD166 ELISA Kit
Gene Names
Alcam; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
Reactivity
Mouse
Synonyms
CD166; N/A; Mouse CD166 ELISA Kit; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; ALCAM elisa kit
Reactivity
Mouse
Specificity
The Mouse CD166 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD166 proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
583
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ALCAM elisa kit
Principle of the Assay: The Mouse CD166 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD166 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CD166 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD166 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD166 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD166 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,092 Da
NCBI Official Full Name
CD166 antigen isoform 1
NCBI Official Synonym Full Names
activated leukocyte cell adhesion molecule
NCBI Official Symbol
Alcam
NCBI Official Synonym Symbols
BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
NCBI Protein Information
CD166 antigen
UniProt Protein Name
CD166 antigen
UniProt Gene Name
Alcam
UniProt Entry Name
CD166_MOUSE
Similar Products
Product Notes
The Mouse ALCAM alcam (Catalog #AAA263101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263101 ELISA Kit recognizes Mouse ALCAM. It is sometimes possible for the material contained within the vial of "CD166, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.