Human Aldolase A, Fructose Bisphosphate ELISA Kit | ALDOA elisa kit
Human Aldolase A, Fructose Bisphosphate ELISA Kit
Reactivity
Human
Synonyms
Aldolase A, Fructose Bisphosphate; N/A; Human Aldolase A, Fructose Bisphosphate ELISA Kit; ALDOA; ALDOA elisa kit
Reactivity
Human
Specificity
Specifically recognize ALDOA, no obvious cross reaction with other analogues
Sequence Length
364
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for ALDOA elisa kit
Background: Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6- bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes, aldolases A, B, and C, which are distinguished by their electrophoretic and catalytic properties. In adult liver, kidney, and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally.Diseases associated with ALDOA include Glycogen Storage Disease Xii and Hemolytic Anemia.Among its related pathways are glycolysis (BioCyc) and Response to elevated platelet cytosolic Ca2+.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ALDOA antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ALDOA antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ALDOA conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ALDOA in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ALDOA antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ALDOA antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ALDOA conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ALDOA in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
aldolase, fructose-bisphosphate A
NCBI Official Synonym Full Names
aldolase, fructose-bisphosphate A
NCBI Official Symbol
aldoa
NCBI Protein Information
aldolase, fructose-bisphosphate A
