Application Data
(ALKBH2 dioxygenase activity assay 1 ug of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 ug, 0.5 ug, 1 ug, 2 ug, 4 ug of ALKBH2 in 50 ul reaction system including 50 mM HEPES-NaOH pH 8.0, 50 ?M Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
ALKBH2 recombinant protein
Recombinant ALKBH2 protein
Gene Names
ALKBH2; ABH2
Synonyms
ALKBH2; N/A; Recombinant ALKBH2 protein; AlkB Homolog 2; Alpha-Ketoglutarate Dependent Dioxygenase; Oxy DC1; ALKBH2 recombinant protein
Host
E Coli
Form/Format
Recombinant ALKBH2 protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 20% glycerol, 0.5mM TCEP.
Protein Species
Human
Tag
His-Tag
Notes
Recombinant ALKBH2 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(ALKBH2 dioxygenase activity assay 1 ug of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 ug, 0.5 ug, 1 ug, 2 ug, 4 ug of ALKBH2 in 50 ul reaction system including 50 mM HEPES-NaOH pH 8.0, 50 ?M Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
Application Data
(ALKBH2 dioxygenase activity assay 1 ug of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 ug, 0.5 ug, 1 ug, 2 ug, 4 ug of ALKBH2 in 50 ul reaction system including 50 mM HEPES-NaOH pH 8.0, 50 ?M Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
SDS-PAGE
(Recombinant ALKBH2 protein 10% SDS-PAGE Coomassie staining MW: 33.1 kDa Purity: >82%)
SDS-PAGE
(Recombinant ALKBH2 protein 10% SDS-PAGE Coomassie staining MW: 33.1 kDa Purity: >82%)
Related Product Information for ALKBH2 recombinant protein
Short Description: Full-length ALKBH2 protein (accession number NP_001001655.1) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of ALKBH2 is 33.1 kDa. Recombinant ALKBH2 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: ALKBH2 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH2 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
Background: ALKBH2 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH2 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
Product Categories/Family for ALKBH2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,083 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 isoform 1
NCBI Official Synonym Full Names
alkB, alkylation repair homolog 2 (E. coli)
NCBI Official Symbol
ALKBH2
NCBI Official Synonym Symbols
ABH2
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; 2OG-Fe(II) oxy DC1; DNA oxidative demethylase ALKBH2; alkylated DNA repair protein alkB homolog 2
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
UniProt Gene Name
ALKBH2
UniProt Synonym Gene Names
ABH2
UniProt Entry Name
ALKB2_HUMAN
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Product Notes
The ALKBH2 alkbh2 (Catalog #AAA60148) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ALKBH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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