Application Data
(ALKBH3 dioxygenase activity 1 ug of ssDNA oligos (5’-AAAGCAG(1mA) ATTCGAAAAAGCGAAA-3’) was incubated with varying concentrations of ALKBH3 in a reaction system including 50 mM HEPES-NaOH pH 8.0, 50 uM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. % reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
ALKBH3 recombinant protein
Recombinant ALKBH3 protein
Gene Names
ALKBH3; ABH3; PCA1; DEPC1; DEPC-1
Synonyms
ALKBH3; N/A; Recombinant ALKBH3 protein; AlkB Homolog 3; Alpha-Ketoglutarate Dependent Dioxygenase; ALKBH3 recombinant protein
Host
E Coli
Form/Format
Recombinant ALKBH3 protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 10% glycerol, 0.5mM TCEP.
Protein Species
Human
Tag
His-Tag
Notes
Recombinant ALKBH3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(ALKBH3 dioxygenase activity 1 ug of ssDNA oligos (5’-AAAGCAG(1mA) ATTCGAAAAAGCGAAA-3’) was incubated with varying concentrations of ALKBH3 in a reaction system including 50 mM HEPES-NaOH pH 8.0, 50 uM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. % reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
Application Data
(ALKBH3 dioxygenase activity 1 ug of ssDNA oligos (5’-AAAGCAG(1mA) ATTCGAAAAAGCGAAA-3’) was incubated with varying concentrations of ALKBH3 in a reaction system including 50 mM HEPES-NaOH pH 8.0, 50 uM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. % reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
SDS-PAGE
(Recombinant ALKBH3 protein 12.5% SDS-PAGE Coomassie staining MW: 36.9 kDa Purity: >90%)
SDS-PAGE
(Recombinant ALKBH3 protein 12.5% SDS-PAGE Coomassie staining MW: 36.9 kDa Purity: >90%)
Related Product Information for ALKBH3 recombinant protein
Short Description: Full-length ALKBH3 protein (accession number NP_631917.1) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of ALKBH3 is 36.9 kDa. Recombinant ALKBH3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: ALKBH3 (AlkB Homolog 3, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It can repair alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. ALKBH3 has a strong preference for single-stranded DNA. It is able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promoting DNA unwinding to generate single-stranded substrate needed for ALKBH3. ALKBH3 also acts on RNA via demethylating N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5-untranslated regions (UTRs) and in the vicinity of start codons. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
Background: ALKBH3 (AlkB Homolog 3, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It can repair alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. ALKBH3 has a strong preference for single-stranded DNA. It is able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promoting DNA unwinding to generate single-stranded substrate needed for ALKBH3. ALKBH3 also acts on RNA via demethylating N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5-untranslated regions (UTRs) and in the vicinity of start codons. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
Product Categories/Family for ALKBH3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,310 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Official Synonym Full Names
alkB, alkylation repair homolog 3 (E. coli)
NCBI Official Symbol
ALKBH3
NCBI Official Synonym Symbols
ABH3; PCA1; DEPC1; DEPC-1
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; alkylated DNA repair protein alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Gene Name
ALKBH3
UniProt Synonym Gene Names
ABH3; DEPC1
UniProt Entry Name
ALKB3_HUMAN
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Product Notes
The ALKBH3 alkbh3 (Catalog #AAA60149) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ALKBH3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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