WB (Western Blot)
(Fig. Western Blot analysis of various cells using NEGR1 Polyclonal Antibody.)
anti-Mullerian hormone ELISA Kit | AMH elisa kit
Human anti-Mullerian hormone, AMH ELISA Kit
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
anti-Mullerian hormone; N/A; Human anti-Mullerian hormone, AMH ELISA Kit; Human anti-Mullerian hormone (AMH) ELISA kit; MIF; MIS; Mullerian inhibiting factor; Mullerian inhibiting substance; AMH elisa kit
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
Specificity
This assay has high sensitivity and excellent specificity for detection of human AMH. No significant cross-reactivity or interference between human AMH and analogues was observed.
Form/Format
Liquid; PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
575
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Applicable Applications for AMH elisa kit
Western Blot (WB), ELISA (EIA)
Application Notes
Optimal working dilutions should be determined experimentally by the investigator.
Suggested starting dilutions are as follows:
WB (1:500-1:2000), ELISA (1:20000).
Not yet tested in other applications.
Suggested starting dilutions are as follows:
WB (1:500-1:2000), ELISA (1:20000).
Not yet tested in other applications.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Store at -20°C. Avoid repeated freeze / thaw cycles
Storage Instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Storage Instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
WB (Western Blot)
(Fig. Western Blot analysis of various cells using NEGR1 Polyclonal Antibody.)
WB (Western Blot)
(Fig. Western Blot analysis of various cells using NEGR1 Polyclonal Antibody.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AMH elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
muellerian-inhibiting factor
NCBI Official Synonym Full Names
anti-Mullerian hormone
NCBI Official Symbol
AMH
NCBI Protein Information
muellerian-inhibiting factor; MIS; anti-Muellerian hormone; muellerian-inhibiting substance
UniProt Protein Name
Muellerian-inhibiting factor
UniProt Gene Name
AMH
UniProt Synonym Gene Names
AMH; MIS
UniProt Entry Name
MIS_BOVIN
Similar Products
Product Notes
The Human, Mouse, Rat AMH amh (Catalog #AAA15347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15347 ELISA Kit recognizes Human, Mouse, Rat AMH. AAA Biotech's anti-Mullerian hormone can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications. Researchers should empirically determine the suitability of the AMH amh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "anti-Mullerian hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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