Chicken Adenosine Monophosphate Activated Protein ELISA Kit | AMPK elisa kit
Chicken Adenosine Monophosphate Activated Protein ELISA Kit
Reactivity
Chicken
Synonyms
Adenosine Monophosphate Activated Protein; N/A; Chicken Adenosine Monophosphate Activated Protein ELISA Kit; AMPK elisa kit
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for AMPK elisa kit
Intended Uses: This AMAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AMAP. This ELISA kit for research use only!
Principle of the Assay: AMAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMAP. Standards or samples are then added to the microtiter plate wells and AMAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMAP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AMAP are added to each well to "sandwich" the AMAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMAP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AMAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMAP. Standards or samples are then added to the microtiter plate wells and AMAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMAP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AMAP are added to each well to "sandwich" the AMAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMAP concentration in each sample is interpolated from this standard curve.
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