Standard Curve (Sample)
Mouse angiotensin I , Ang-I ELISA Kit | Ang- elisa kit
Mouse angiotensin I , Ang-I ELISA Kit
Reactivity
Mouse
Synonyms
angiotensin I , Ang-I; N/A; Mouse angiotensin I , Ang-I ELISA Kit; Mouse angiotensin I; Ang-I ELISA Kit; angiotensin I; Ang-; Ang- elisa kit
Reactivity
Mouse
Sequence Length
10
Samples
Serum, plasma
Detection Range
0.097 pg/ml-400 pg/ml
Sensitivity
0.097 pg/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Ang- elisa kit
Introduction: Angiotensin causes blood vessels to constrict, and drives blood pressure up. It is part of the renin-angiotensin system, which is a major target for drugs that lower blood pressure. Angiotensin also stimulates the release of aldosterone from the adrenal cortex. Aldosterone promotes sodium retention in the distal nephron, which also drives blood pressure up. Angiotensin I is formed by the action of renin on angiotensinogen. Renin is produced in the kidneys in response to both decreased intra-renal blood pressure at the juxtaglomerular cells, or decreased delivery of Na+ and Cl- to the macula densa. If more Na+ is sensed, renin release is decreased. Renin cleaves the peptide bond between the leucine and valine residues on angiotensinogen, creating the ten amino acid peptide (des-Asp) angiotensin I. Angiotensin I appears to have no biological activity and exists solely as a precursor to angiotensin II. Angiotensin I is converted to angiotensin II through removal of two terminal residues by the enzyme Angiotensin-converting enzyme, which is found predominantly in the capillaries of the lung.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ang-I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ang-Iand Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Ang-I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Ang-Iin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ang-I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ang-Iand Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Ang-I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Ang-Iin the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
angiotensin I, ANG I
Similar Products
Product Notes
The Mouse Ang- (Catalog #AAA79905) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79905 ELISA Kit recognizes Mouse Ang-. It is sometimes possible for the material contained within the vial of "angiotensin I , Ang-I, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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