ELISA
(ELISA using A1227 and Norovirus GII.4 VLP. Binding curves of the rabbit IgG chimeric version of the Anti-Norovirus capsid protein VP1 antibody A1227 and isotype control (yellow line) to an ELISA plate coated with Norovirus GII.4 VLP at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.)
Norovirus capsid protein VP1 Antibody | anti-VP1 antibody
Anti-Norovirus capsid protein VP1 [A1227]
Reactivity
Norovirus
Applications
ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Norovirus capsid protein VP1; N/A; Anti-Norovirus capsid protein VP1 [A1227]; VP1; CP; p59; soluble capsid protein; Protein 30k; p30; Norwalk virus; anti-VP1 antibody
Reactivity
Norovirus
Isotype
Mouse IgG1 kappa
Clone Number
A1227
Specificity
This antibody shows broadly binding but no blocking activity toward multiple GI and GII strains such as GI.I, GII.2, and GII.17. This antibody recognizes a highly conserved epitope located at an occluded site on the norovirus capsid, at the interface between the P domain and shell domain of the Norovirus, which explain its high level of cross-reactivity. Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-VP1 antibody
ELISA
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original human IgG1 format for improved compatibility with existing reagents assays and techniques
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA
(ELISA using A1227 and Norovirus GII.4 VLP. Binding curves of the rabbit IgG chimeric version of the Anti-Norovirus capsid protein VP1 antibody A1227 and isotype control (yellow line) to an ELISA plate coated with Norovirus GII.4 VLP at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.)
ELISA
(ELISA using A1227 and Norovirus GII.4 VLP. Binding curves of the rabbit IgG chimeric version of the Anti-Norovirus capsid protein VP1 antibody A1227 and isotype control (yellow line) to an ELISA plate coated with Norovirus GII.4 VLP at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.)
Related Product Information for anti-VP1 antibody
The original antibody was isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine.
Describes the generation of this antibody and study of its crystal structure.
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Describes the generation of this antibody and study of its crystal structure.
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-VP1 antibody
References
Lindesmith et al.
Immunity. 2019 Jun 18;50(6):1530-1541.
Immunity. 2019 Jun 18;50(6):1530-1541.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
198,694 Da
NCBI Official Full Name
nonstructural polyprotein
NCBI Official Symbol
NoVGI_gp1
NCBI Protein Information
nonstructural polyprotein; p48; NTPase; p22; VPg; Pro; RdRp
UniProt Protein Name
Genome polyprotein
UniProt Gene Name
ORF1
UniProt Synonym Gene Names
3CLpro; RdRp
UniProt Entry Name
POLG_NVN68
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Product Notes
The VP1 orf1 (Catalog #AAA72329) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Norovirus capsid protein VP1 [A1227] reacts with Norovirus and may cross-react with other species as described in the data sheet. AAA Biotech's Norovirus capsid protein VP1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the VP1 orf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Norovirus capsid protein VP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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