CLOSTRIDIUM DIFFICILE TOXOID A Antigen
CLOSTRIDIUM DIFFICILE TOXOID A
Applications
ELISA
Purity
>95% by SDS-PAGE
Synonyms
CLOSTRIDIUM DIFFICILE TOXOID A; N/A; CLOSTRIDIUM DIFFICILE TOXOID A antigen
Purity/Purification
>95% by SDS-PAGE
Form/Format
Purified
Inactivated toxoid derived from purified Clostridium difficile Toxin A - lyophilised
Inactivated toxoid derived from purified Clostridium difficile Toxin A - lyophilised
Concentration
Approximate Protein Concentration: 0.2 mg/ml after reconstitution (varies by lot)
Applicable Applications for CLOSTRIDIUM DIFFICILE TOXOID A antigen
ELISA
Reconstitution
Reconstitute with 250 ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution.
Preparation
Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2ug/ml compared to active toxin lethal dose of 0.2ng/ml.
Buffer Solution
0.05M HEPES, 0.15M NaCl, 5% Sucrose
Target Species
Bacterial
Preservative Stabilizers
None present
Preparation and Storage
Prior to reconstitution store at 4°C.
After reconstitution store at 4°C or at -20°C for long term storage.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Shelf life: 1 month from date of reconstitution if stored at 4°C.
After reconstitution store at 4°C or at -20°C for long term storage.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.
Shelf life: 1 month from date of reconstitution if stored at 4°C.
Related Product Information for CLOSTRIDIUM DIFFICILE TOXOID A antigen
Clostridium difficile is a Gram-positive bacteria naturally present in the gut of some children and very few adults. Occasionally, the use of antibiotics to treat other infections may lead to C. difficile infection and severe diarrhoea and fever. Symptoms are caused by toxin A (an enterotoxin) and toxin B (a cytotoxin).
MBS239268 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin A. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.
For a preparation of Toxoid B, see MBS239267.
MBS239268 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin A. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.
For a preparation of Toxoid B, see MBS239267.
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Product Notes
The CLOSTRIDIUM DIFFICILE TOXOID A (Catalog #AAA50655) is an Antigen and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CLOSTRIDIUM DIFFICILE TOXOID A can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CLOSTRIDIUM DIFFICILE TOXOID A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLOSTRIDIUM DIFFICILE TOXOID A, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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