Human Annexin A2 ELISA Kit | ANXA2 elisa kit

Human Annexin A2 ELISA Kit

Cat. Num. AAA16625

• Gene Names: ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
• Reactivity: Human
• Synonyms: Annexin A2; Human Annexin A2 ELISA Kit; ANXA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANXA2. No significant cross-reactivity or interference between ANXA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ANXA2 elisa kit

Principle of the Assay: ANXA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANXA2 antibody and an ANXA2-HRP conjugate. The assay sample and buffer are incubated together with ANXA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA2 concentration since ANXA2 from samples and ANXA2-HRP conjugate compete for the anti-ANXA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA2 from the sample, fewer sites are left to bind ANXA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ANXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ANXA2 elisa kit

Cell Biology

References

Serum annexin A2 levels in patients with colon cancer in comparison to healthy controls and in relation to tumor pathology. Ercument Gurluler, Osman Serhat Guner, Latif Volkan Tumay, Nurten Turkel Kucukmetin, Banu Hizli, and Abdullah Zorluoglu (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 209862831
• NCBI GeneID: 302
• NCBI Accession #: NP_001129487.1
• NCBI GenBank Nucleotide #: NM_001136015.2
• Molecular Weight: 40,411 Da
• NCBI Official Full Name: annexin A2 isoform 2
• NCBI Official Synonym Full Names: annexin A2
• NCBI Official Symbol: ANXA2
• NCBI Official Synonym Symbols: P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
• NCBI Protein Information: annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV; epididymis secretory protein Li 270
• UniProt Protein Name: Annexin A2
• UniProt Gene Name: ANXA2
• UniProt Synonym Gene Names: ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV
• UniProt Entry Name: ANXA2_HUMAN