Mouse Advanced oxidation protein products ELISA Kit | AOPP elisa kit

Mouse Advanced oxidation protein products ELISA Kit

Cat. Num. AAA86376

• Reactivity: Mouse
• Synonyms: Advanced oxidation protein products; N/A; Mouse Advanced oxidation protein products ELISA Kit; AOPP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of AOPP. No significant cross-reactivity or interference between AOPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AOPP and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for AOPP elisa kit

Principle of the Assay: AOPP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AOPP antibody and an AOPP-HRP conjugate. The assay sample and buffer are incubated together with AOPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOPP concentration since AOPP from samples and AOPP-HRP conjugate compete for the anti-AOPP antibody binding site. Since the number of sites is limited, as more sites are occupied by AOPP from the sample, fewer sites are left to bind AOPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOPP concentration in each sample is interpolated from this standard curve.

Intended Uses: This AOPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AOPP. This ELISA kit for research use only, not for therapeutic or test applications!