Rat Activated Protein C ELISA Kit | APC elisa kit

Rat Activated Protein C ELISA Kit

Cat. Num. AAA32381

• Gene Names: APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• Reactivity: Rat
• Synonyms: Activated Protein C; N/A; Rat Activated Protein C ELISA Kit; APC elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat APC and analogues was observed.
• Sequence Length: 2843
• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 ng/ml - 2000 ng/ml.
• Sensitivity: 10 ng/ml.

• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated APC concentrations in Rat serum, plasma and other body fluids. Using Purified Rat APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for APC elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated APC concentrations in Rat serum, plasma and other body fluids. Using Purified Rat APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NM_000038.5
• UniProt Accession #: P25054; Q15162; Q15163; Q93042; D3DT03
• Molecular Weight: 311,646 Da
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
• UniProt Protein Name: Adenomatous polyposis coli protein
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN