Apolipoprotein A-I/APOA1 Recombinant Protein | APOA1 recombinant protein

Apolipoprotein A-I/APOA1 Protein, Mouse, Recombinant (hFc Tag), HPLC-verified

Cat. Num. AAA259256

• Gene Names: Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• Purity: >= 90% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Synonyms: Apolipoprotein A-I/APOA1; N/A; Apolipoprotein A-I/APOA1 Protein, Mouse, Recombinant (hFc Tag), HPLC-verified; apolipoprotein A-I; 1-Sep Protein, Mouse; 2-Sep Protein, Mouse; 42248 Protein, Mouse; 42249 Protein, Mouse; Alp-1 Protein, Mouse; apo-AI Protein, Mouse; Apoa-1 Protein, Mouse; apoA-I Protein, Mouse; Brp-14 Protein, Mouse; Ltw-1 Protein, Mouse; Lvtw-1 Protein, Mouse; APOA1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 90% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM Nacl, 10% Glycerol, 10% trehalose, 0.02% Tween 80, pH 8.5.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: Mouse
• Predicted N Terminal: Asp 25
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Mouse APOA1 (Q00623)(Asp25-Gln264) was expressed with the Fc region of human IgG1 at the C-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

Application Data

Related Product Information for APOA1 recombinant protein

Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

References

Toptas B, et al. (2011) Comparison of lipid profiles with APOA1 MspI polymorphism in obese children with hyperlipidemia. In Vivo. 25(3): 425-30.
Haase CL, et al. (2011) Mutation in APOA1 predicts increased risk of ischaemic heart disease and total mortality without low HDL cholesterol levels. J Intern Med. 270(2): 136-46.
Wu Z, et al. (2011) The low resolution structure of ApoA1 in spherical high density lipoprotein revealed small angle neutron scattering. J Biol Chem. 286(14): 12495-508.

NCBI and Uniprot Product Information

• NCBI GI #: 160333304
• NCBI GeneID: 11806
• NCBI Accession #: NP_033822.2
• NCBI GenBank Nucleotide #: NM_009692.4
• UniProt Accession #: Q00623; O08855; O09042; Q8BPD5
• Molecular Weight: 30,616 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_MOUSE