Human Apolipoprotein A4 ELISA Kit | APOA4 elisa kit
Human Apolipoprotein A4 ELISA Kit
Reactivity
Human
Synonyms
Apolipoprotein A4; N/A; Human Apolipoprotein A4 ELISA Kit; APOA4 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
10-250ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for APOA4 elisa kit
Intended Uses: This ApoA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ApoA4.
Principle of the Assay: ApoA4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoA4. Standards or samples are then added to the microtiter plate wells and ApoA4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoA4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoA4 are added to each well to "sandwich" the ApoA4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoA4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoA4 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ApoA4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoA4. Standards or samples are then added to the microtiter plate wells and ApoA4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoA4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoA4 are added to each well to "sandwich" the ApoA4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoA4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoA4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for APOA4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,399 Da
NCBI Official Full Name
apolipoprotein A-IV
NCBI Official Synonym Full Names
apolipoprotein A-IV
NCBI Official Symbol
APOA4
NCBI Protein Information
apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
UniProt Protein Name
Apolipoprotein A-IV
UniProt Gene Name
APOA4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV
UniProt Entry Name
APOA4_HUMAN
