Human apolipoprotein B (including Ag (x) antigen) ELISA Kit | APOB elisa kit

Human apolipoprotein B100, Apo-B100 ELISA Kit

Cat. Num. AAA79754

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Human
• Synonyms: apolipoprotein B (including Ag (x) antigen); N/A; Human apolipoprotein B100, Apo-B100 ELISA Kit; Human apolipoprotein B100 (Apo-B100) ELISA Kit; FLDB; LDLCQ4; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B48; Human apolipoprotein B100 (Apo-B100); APOB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human APOB. No significant cross-reactivity or interference between Human APOB and analogues was observed.
• Sequence Length: 4563
• Samples: Serum, plasma, cell culture supernates, saliva, urine
• Detection Range: 78 ng/ml-5000 ng/ml
• Sensitivity: 19.5 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apolipoprotein
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for APOB elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APO-B100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated polyclonal antibody preparation specific for APO-B100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain APO-B100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of APO-B100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN