Standard Curve (Sample)
Mouse Apolipoprotein B-100 ELISA Kit | Apob elisa kit
Mouse Apolipoprotein B-100 ELISA Kit
Gene Names
Apob; apob-48; AI315052; apob-100; Apo B-100
Reactivity
Mouse
Synonyms
Apolipoprotein B-100; N/A; Mouse Apolipoprotein B-100 ELISA Kit; Apo B-100; Apob; Apob elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural Mouse Apo B-100. No significant cross-reactivity or interference was observed.
Sequence Length
4505
Assay Type
Sandwich
Detection Range
31.2-2000 pg/mL
Sensitivity
15.78 pg/mL
Intra-Assay CV
<=7.9%
Inter-Assay CV
<=10.1%
Recovery
102%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Apob elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of Mouse apolipoprotein b-100,Apo B-100 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo B-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo B-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo B-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Apo B-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo B-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo B-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo B-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Apo B-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
509,432 Da
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
Apob
NCBI Official Synonym Symbols
apob-48; AI315052; apob-100; Apo B-100
NCBI Protein Information
apolipoprotein B-100
UniProt Protein Name
Apolipoprotein B-100
UniProt Gene Name
Apob
UniProt Synonym Gene Names
Apo B-100; Apo B-48
UniProt Entry Name
APOB_MOUSE
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Product Notes
The Mouse Apob apob (Catalog #AAA194909) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA194909 ELISA Kit recognizes Mouse Apob. It is sometimes possible for the material contained within the vial of "Apolipoprotein B-100, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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