Apolipoprotein-D Recombinant Protein | APOD recombinant protein

Recombinant Human Apolipoprotein-D

Cat. Num. AAA38585

• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Apolipoprotein-D; N/A; Recombinant Human Apolipoprotein-D; Apo D Human; Apolipoprotein-D Human Recombinant; Apolipoprotein D; Apo-D; ApoD; APOD recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: Filtered (0.4 um) and lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH 7.5.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH
• Sequence Length: 189

• Solubility: It is recommended to add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for APOD recombinant protein

Description: Apolipoprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82kDa. The protein a.a sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.The Following gene modifications were made:Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein's solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques.

Introduction: Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid. Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer's disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.

Product Categories/Family for APOD recombinant protein

Cytokines and Growth Factors; Cytokines; Apolipoprotein

NCBI and Uniprot Product Information

• NCBI GI #: 4502163
• NCBI GeneID: 347
• NCBI Accession #: NP_001638.1
• NCBI GenBank Nucleotide #: NM_001647.3
• UniProt Accession #: P05090; Q6IBG6; B2R579; D3DNW6
• Molecular Weight: 21,276 Da
• NCBI Official Full Name: apolipoprotein D
• NCBI Official Synonym Full Names: apolipoprotein D
• NCBI Official Symbol: APOD
• NCBI Protein Information: apolipoprotein D; apo-D
• UniProt Protein Name: Apolipoprotein D
• UniProt Gene Name: APOD
• UniProt Synonym Gene Names: Apo-D; ApoD
• UniProt Entry Name: APOD_HUMAN