Standard Curve (Sample)
Porcine A Proliferation inducing ligand ELISA Kit | APRIL elisa kit
Porcine A Proliferation inducing ligand ELISA Kit
Gene Names
TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
Reactivity
Porcine
Synonyms
A Proliferation inducing ligand; N/A; Porcine A Proliferation inducing ligand ELISA Kit; APRIL elisa kit
Reactivity
Porcine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for APRIL elisa kit
Intended Uses: This APIL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse APIL. This ELISA kit for research use only!
Principle of the Assay: APIL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-APIL antibody and an APIL-HRP conjugate. The assay sample and buffer are incubated together with APIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APIL concentration since APIL from samples and APIL-HRP conjugate compete for the anti-APIL antibody binding site. Since the number of sites is limited, as more sites are occupied by APIL from the sample, fewer sites are left to bind APIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APIL concentration in each sample is interpolated from this standard curve.
Principle of the Assay: APIL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-APIL antibody and an APIL-HRP conjugate. The assay sample and buffer are incubated together with APIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APIL concentration since APIL from samples and APIL-HRP conjugate compete for the anti-APIL antibody binding site. Since the number of sites is limited, as more sites are occupied by APIL from the sample, fewer sites are left to bind APIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APIL concentration in each sample is interpolated from this standard curve.
Product Categories/Family for APRIL elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,589 Da
NCBI Official Full Name
APRIL
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13
NCBI Official Symbol
TNFSF13
NCBI Official Synonym Symbols
APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13
UniProt Gene Name
TNFSF13
UniProt Synonym Gene Names
APRIL; TALL2; ZTNF2; UNQ383/PRO715; APRIL; TALL-2; TRDL-1
UniProt Entry Name
TNF13_HUMAN
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Product Notes
The Porcine APRIL tnfsf13 (Catalog #AAA93605) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA93605 ELISA Kit recognizes Porcine APRIL. It is sometimes possible for the material contained within the vial of "A Proliferation inducing ligand, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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