SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Recombinant Escherichia coli Aquaporin Z (aqpZ) Recombinant Protein | AQPZ recombinant protein
Recombinant Escherichia coli Aquaporin Z (aqpZ)(Active)
Gene Names
aqpZ; bniP; ECK0866; JW0859
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Recombinant Escherichia coli Aquaporin Z (aqpZ); N/A; Recombinant Escherichia coli Aquaporin Z (aqpZ)(Active); Bacterial nodulin-like intrinsic protein; AQPZ recombinant protein
Host
E coli (in vitro)
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
Lyophilized powder. Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0
Sequence
MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
Sequence Length
1-231aa; Full Length
Species
Escherichia coli (strain K12)
Tag
N-terminal 6xHis-tagged
Transmembrane Domain
6TM
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5ug/ml can bind E coli ytfE, the EC50 of E coli ytfE protein is 197.90-259.70ug/ml.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Bioactivity
Bioactivity
Related Product Information for AQPZ recombinant protein
Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity
Product Categories/Family for AQPZ recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,703 Da
NCBI Official Full Name
aquaporin Z
NCBI Official Symbol
aqpZ
NCBI Official Synonym Symbols
bniP; ECK0866; JW0859
NCBI Protein Information
aquaporin Z
UniProt Protein Name
Aquaporin Z
UniProt Gene Name
aqpZ
UniProt Synonym Gene Names
bniP
UniProt Entry Name
AQPZ_ECOLI
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Product Notes
The AQPZ aqpz (Catalog #AAA279388) is a Recombinant Protein produced from E coli (in vitro) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MFRKLAAECF GTFWLVFGGC GSAVLAAGFP ELGIGFAGVA LAFGLTVLTM AFAVGHISGG HFNPAVTIGL WAGGRFPAKE VVGYVIAQVV GGIVAAALLY LIASGKTGFD AAASGFASNG YGEHSPGGYS MLSALVVELV LSAGFLLVIH GATDKFAPAG FAPIAIGLAL TLIHLISIPV TNTSVNPARS TAVAIFQGGW ALEQLWFFWV VPIVGGIIGG LIYRTLLEKR D. It is sometimes possible for the material contained within the vial of "Recombinant Escherichia coli Aquaporin Z (aqpZ), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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