Porcine Androgen Receptor ELISA Kit | AR elisa kit

Porcine Androgen Receptor ELISA Kit

Cat. Num. AAA89498

• Gene Names: AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Porcine
• Synonyms: Androgen Receptor; N/A; Porcine Androgen Receptor ELISA Kit; AR elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of AR. No significant cross-reactivity or interference between AR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AR and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue
• Sensitivity: 0.1 ng/mL

• Intended Uses: This AR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for AR elisa kit

Principle of the Assay: AR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AR antibody and an AR-HRP conjugate. The assay sample and buffer are incubated together with AR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AR concentration since AR from samples and AR-HRP conjugate compete for the anti-AR antibody binding site. Since the number of sites is limited, as more sites are occupied by AR from the sample, fewer sites are left to bind AR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AR concentration in each sample is interpolated from this standard curve.

Intended Uses: This AR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AR. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for AR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 178882
• NCBI GeneID: 367
• Molecular Weight: 44,643 Da
• NCBI Official Full Name: androgen receptor
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
• UniProt Protein Name: Androgen receptor
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4
• UniProt Entry Name: ANDR_HUMAN