Artemin ELISA Kit | ARTN elisa kit

Human Artemin PicoKine ELISA Kit

Cat. Num. AAA124860

• Gene Names: ARTN; ART; EVN; NBN; ENOVIN
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: Artemin; N/A; Human Artemin PicoKine ELISA Kit; Enovin; Neublastin; ARTN; EVN; ARTN elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human Artemin
• Sequence Length: 228
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <2pg/ml

• Applicable Applications for ARTN elisa kit: ELISA
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for ARTN elisa kit

Principle of the Assay: Human Artemin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Artemin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A108-G220) and test samples are added to the wells, a biotinylated detection biotinylated polyclonal antibody from goat specific for Artemin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Artemin amount of sample captured in plate.

Background: Artemin, also known as enovin or neublastin, is a protein that in humans is encoded by the ARTN gene. This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 209977029
• NCBI GeneID: 9048
• NCBI Accession #: NP_001129687.1
• NCBI GenBank Nucleotide #: NM_001136215.1
• UniProt Accession #: Q5T4W7; O95441; O96030; Q6P6A3; D3DPY1; D3DPY3
• Molecular Weight: 23,616 Da
• NCBI Official Full Name: artemin isoform 3
• NCBI Official Synonym Full Names: artemin
• NCBI Official Symbol: ARTN
• NCBI Official Synonym Symbols: ART; EVN; NBN; ENOVIN
• NCBI Protein Information: artemin
• UniProt Protein Name: Artemin
• UniProt Gene Name: ARTN
• UniProt Synonym Gene Names: EVN