Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ASH1L recombinant protein
Recombinant ASH1L (2407-2579) protein
Gene Names
ASH1L; ASH1; KMT2H; ASH1L1
Synonyms
ASH1L; N/A; Recombinant ASH1L (2407-2579) protein; ASH1; KMT2H; ASH1L1; ASH1L recombinant protein
Host
E Coli
Form/Format
Recombinant ASH1L (2407-2579) protein was expressed in E Coli cells and is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, and 10% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant ASH1L (2407-2579) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant ASH1L (2407-2579) protein gel. ASH1L (2407-2579) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 23.2 kDa Purity: > 85%)
SDS-PAGE
(Recombinant ASH1L (2407-2579) protein gel. ASH1L (2407-2579) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 23.2 kDa Purity: > 85%)
Related Product Information for ASH1L recombinant protein
Short Description: The peptide corresponding to amino acids 2407-2579 that contains the bromodomain sequences of ASH1L (accession number NP_060959.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 25.6 kDa. ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy. Recombinant ASH1L (2407-2579) can be used in binding assays and inhibitor screening.
Background: ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy.
Background: ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy.
Product Categories/Family for ASH1L recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
332,211 Da
NCBI Official Full Name
Homo sapiens ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), mRNA
NCBI Official Synonym Full Names
ash1 (absent, small, or homeotic)-like (Drosophila)
NCBI Official Symbol
ASH1L
NCBI Official Synonym Symbols
ASH1; KMT2H; ASH1L1
NCBI Protein Information
histone-lysine N-methyltransferase ASH1L; ASH1-like protein; absent small and homeotic disks protein 1 homolog; lysine N-methyltransferase 2H; probable histone-lysine N-methyltransferase ASH1L
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Product Notes
The ASH1L (Catalog #AAA59687) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ASH1L, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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