6-Phosphofructokinase (PFK) Activity Assay Kit | PFK assay kit
6-Phosphofructokinase (PFK) Activity Colorimetric Assay Kit
Synonyms
6-Phosphofructokinase (PFK) Activity; N/A; 6-Phosphofructokinase (PFK) Activity Colorimetric Assay Kit; 6-Phosphofructokinase; PFK; Colorimetric; PFK Assay; PFK assay kit
Samples
Serum, Plasma, Animal and Plant Tissues, Cells, Cell culture supernatant, Bacteria
Usage Notes
Do not mix or substitute reagents or materials from other kit lots or vendors.
It is advisable to measure serum potassium as soon as possible after blood collection.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
It is advisable to measure serum potassium as soon as possible after blood collection.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Preparation and Storage
Store at 4 degree C and protect from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for PFK assay kit
Principle of the Assay: Phosphofructokinase (PFK, EC 2.7.1.11) is widely present in animals, plants, microorganisms and cultured cells. It is important in regulating the process of fermentation, by which one molecule of the simple sugar glucose is broken down to two molecules of pyruvic acid. The enzyme, one of a class called transferases, catalyzes one of several specific reactions involved in this breakdown—the formation of fructose-1,6-diphosphate and adenosine diphosphate (ADP) from fructose-6-phosphate and adenosine triphosphate (ATP); its activity is sensitive to the ATP/ADP ratio in the cell. PFK is one of the key regulatory enzymes in the glycolysis process. CheKine™ Micro 6-Phosphofructokinase (PFK) Activity Assay Kit provides a convenient tool for sensitive detection of PFK Activity. The principle is that PFK catalyzes fructose-6-phosphate and ATP to produce fructose-1,6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. NADH has a maximum absorption peak detected at about 340 nm. The enzyme activity of PFK was calculated by detecting the rate of decrease in absorption at 340 nm.
Product Categories/Family for PFK assay kit
Similar Products
Product Notes
The PFK (Catalog #AAA332510) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "6-Phosphofructokinase (PFK) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.