Standard Curve (Sample)
96-well Cellular Senescence Assay (SA beta-Gal Activity) Assay Kit
96-well Cellular Senescence Assay (SA beta-Gal Activity)
Synonyms
96-well Cellular Senescence Assay (SA beta-Gal Activity); N/A; 96-well Cellular Senescence Assay (SA b-Gal Activity); 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit
Preparation and Storage
Store SA-beta-gal substrate solution protected from light at -20 degree C. Store all other components at room temperature.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit
Background: Normal primary cells proliferate in culture for a limited number of population doublings prior to undergoing terminal growth arrest and acquiring a senescent phenotype. This finite life span correlates with the age of the organism and with the life expectancy of the species from which the cells were obtained; such that the older the age or the shorter the life span, the less the ability of the cells to undergo population doubling. Senescent cells are characterized by an irreversible G1 growth arrest involving the repression of genes that drive cell cycle progression and the upregulation of cell cycle inhibitors like p16INK4a, p53, and its transcriptional target, p21CIP1. They are resistant to mitogeninduced proliferation, and assume a characteristic enlarged, flattened morphology. Research into the pathways that positively regulate senescence and ways cells bypass senescence is therefore critical in understanding carcinogenesis. Normal cells have several mechanisms in place to protect against uncontrolled proliferation and tumorigenesis.
Product Categories/Family for 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit
References
1. Current Protocols in Molecular Biology, John Wiley & Sons Press.
2. Campisi, J. (2000) In Vivo 14, 183-188.
3. Dimri, G. P., X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocke, and J. Campisi. (1995) Proc. Natl. Acad. Sci. USA 92:9363-9367.
1. Lu, J. et al. (2014). SIRT1 counteracted the activation of STAT3 and NF-kappaB to repress the gastric cancer growth. Int J Clin Exp Med. 7:5050-5058.
2. Malhotra, D. et al. (2010). Global mapping of binding sites for Nrf2 identifies novel targets in cell survival response through ChIP-Seq profiling and network analysis. Nucleic Acids Res. 10.1093/nar/gkq212.
2. Campisi, J. (2000) In Vivo 14, 183-188.
3. Dimri, G. P., X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocke, and J. Campisi. (1995) Proc. Natl. Acad. Sci. USA 92:9363-9367.
1. Lu, J. et al. (2014). SIRT1 counteracted the activation of STAT3 and NF-kappaB to repress the gastric cancer growth. Int J Clin Exp Med. 7:5050-5058.
2. Malhotra, D. et al. (2010). Global mapping of binding sites for Nrf2 identifies novel targets in cell survival response through ChIP-Seq profiling and network analysis. Nucleic Acids Res. 10.1093/nar/gkq212.
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Product Notes
The 96-well Cellular Senescence Assay (SA beta-Gal Activity) (Catalog #AAA44557) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "96-well Cellular Senescence Assay (SA beta-Gal Activity), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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