Standard Curve (Sample)
ADP assay kit
ADP Assay Kit
Synonyms
ADP; N/A; ADP Assay Kit; ADP assay kit
Detection Method
Fluorometric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADP assay kit
Background: Adenosine diphosphate (ADP) is an organic molecule that plays a role in energy flow for various cell processes such as enzymatic reactions leading to nerve impulses, muscle contraction, and synthesis of chemicals. ADP is found in all life forms and consists of three components: a nitrogenous base (adenine), the sugar ribose, and the diphosphate group. It is consumed in serial enzymatic reactions that produce ATP such as glycolysis or oxidative phosphorylation. In plants, sunlight is converted from ADP to ATP through a process known as photosynthesis. When used up metabolically, ATP is converted either to adenosine diphosphate (ADP) or to adenosine monophosphate (AMP). The ADP Assay Kit is a simple fluorometric assay that measures the amount of total ADP present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, ADP standards, and unknown samples. Sample ADP concentrations are determined by comparison with a known ADP standard. The kit has a detection sensitivity limit of 3.1 uM ADP.
Principle of the Assay: The ADP Assay Kit measures total ADP within biological samples. ADP and phosphoenolpyruvate (PEP) are converted by pyruvate kinase to ATP and pyruvate. Pyruvate is oxidized by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of ATP standard within the 96-well microtiter plate format. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Principle of the Assay: The ADP Assay Kit measures total ADP within biological samples. ADP and phosphoenolpyruvate (PEP) are converted by pyruvate kinase to ATP and pyruvate. Pyruvate is oxidized by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of ATP standard within the 96-well microtiter plate format. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
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Product Notes
The ADP (Catalog #AAA44695) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ADP, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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