Standard Curve (Sample)
Aspartate Aminotransferase (AST) Activity Assay Kit | AST assay kit
Aspartate Aminotransferase (AST) Activity Assay Kit (Colorimetric)
Synonyms
Aspartate Aminotransferase (AST) Activity; N/A; Aspartate Aminotransferase (AST) Activity Assay Kit (Colorimetric); AST assay kit
Preparation and Storage
Upon receipt, store the AST Positive Control and 10X Assay Buffer at 4 degree C. Store the remaining components at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AST assay kit
Principle of the Assay: The Aspartate Aminotransferase (AST) Assay Kit measures AST activity through a series of enzyme driven reactions. AST within samples reacts with aspartate to transfer an amino group to alpha-ketoglutarate, producing glutamate and oxaloacetate. Glutamate is then detected with the colorimetric probe. Samples and standards are incubated for 60-120 minutes and then read with a standard 96-well specrophotometric plate reader (450 nm). The AST activity is directly proportional to the amount of glutamate generated in the reaction. Sample AST levels are determined by comparison with the known glutamate standards.
Background/Introduction: Aspartate Aminotransferase (AST), also known as serum glutamic-oxaloacetic transaminase (GOT, SGOT), is an enzyme that catalyzes the transfer of an alpha amino group from aspartate to alpha-ketoglutarate, thereby producing glutamate and oxaloacetic acid (Figure 1). The reaction is important in amino acid biosynthesis and degradation. This pyridoxal phosphate (PLP) cofactor dependent transaminase is found in various tissues such as liver, heart, kidneys, brain, muscles, and red blood cells. AST is usually measured as a clinical marker of liver function to ascertain liver health in blood panel testing. Hepatocellular injury often leads to an increase in AST levels, which are usually measured in units per liter (U/L). Acute renal disease, muskoskeletal afflictions, myocardial infarction, pancreatitis, anemia, burns, and trauma have all displayed high levels of AST. The Aspartate Aminotransferase (AST) Assay Kit is a simple colorimetric assay that measures the activity of AST present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. An AST positive control is also provided.
Background/Introduction: Aspartate Aminotransferase (AST), also known as serum glutamic-oxaloacetic transaminase (GOT, SGOT), is an enzyme that catalyzes the transfer of an alpha amino group from aspartate to alpha-ketoglutarate, thereby producing glutamate and oxaloacetic acid (Figure 1). The reaction is important in amino acid biosynthesis and degradation. This pyridoxal phosphate (PLP) cofactor dependent transaminase is found in various tissues such as liver, heart, kidneys, brain, muscles, and red blood cells. AST is usually measured as a clinical marker of liver function to ascertain liver health in blood panel testing. Hepatocellular injury often leads to an increase in AST levels, which are usually measured in units per liter (U/L). Acute renal disease, muskoskeletal afflictions, myocardial infarction, pancreatitis, anemia, burns, and trauma have all displayed high levels of AST. The Aspartate Aminotransferase (AST) Assay Kit is a simple colorimetric assay that measures the activity of AST present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. An AST positive control is also provided.
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Product Notes
The AST (Catalog #AAA44673) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase (AST) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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