Application Data
Ca2+-ATPase assay kit
Ca2+-ATPase Activity Assay Kit
Synonyms
Ca2+-ATPase; N/A; Ca2+-ATPase Activity Assay Kit; Ca2+-ATPase assay kit
Form/Format
Ultrapure water, Normal saline (0.9% NaCl)
Samples
Animal tissue samples
Detection Range
1.18-286.43U/kg wet weight
Sensitivity
1.18U/kgwetweight
Intra-assay Precision
6
Inter-assay Precision
8
Preparation and Storage
Store at 2-8 degree C
Application Data
Application Data
Related Product Information for Ca2+-ATPase assay kit
Background: ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca2+ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca2+ -ATPase on the cell membrane, and Ca2+ -ATPase consumes ATP to generate the energy needed for ion transport.
Principle of the Assay: ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+-ATPase activity was inhibited, while in the sample system, Ca2+-ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+-ATPase during the incubation time. The activity of Ca2+-ATPase was determined by inorganic phosphorus production.
Principle of the Assay: ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+-ATPase activity was inhibited, while in the sample system, Ca2+-ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+-ATPase during the incubation time. The activity of Ca2+-ATPase was determined by inorganic phosphorus production.
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Product Notes
The Ca2+-ATPase (Catalog #AAA178085) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ca2+-ATPase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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