Standard Curve (Sample)
Caspase-9 Assay Kit | CASP9 assay kit
Caspase-9 Assay Kit (Colorimetric)
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Synonyms
Caspase-9; N/A; Caspase-9 Assay Kit (Colorimetric); CASP9 assay kit
Sequence Length
266
Samples
Cells, Animal Tissues
Preparation and Storage
Stable for at least 12 months at recommended temperature from date of shipment.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CASP9 assay kit
Principle of the Assay: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-9 (also known as ICE-LAP6 and Mch6) is a member of the caspase-family of cysteine proteases. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis. Caspase 9 kit (colorimetric) based on caspase 9 can catalyze the substrate Ac-LEHD-pNA (acetyl-L-Leu-Glu-His-Asp p-nitroanilide) to produce yellow nitroaniline (pNA), The pNA is strongly absorbed around 405 nm, so that the caspase 9 activity can be measured by measuring the absorbance.
Product Categories/Family for CASP9 assay kit
NCBI and Uniprot Product Information
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Product Notes
The CASP9 (Catalog #AAA332449) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Caspase-9, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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