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product-image-AAA174668_SC15.jpg Standard Curve (Sample)

Catalase (CAT) Assay Kit | CAT assay kit

Catalase (CAT) Colorimetric Assay Kit

Synonyms
Catalase (CAT); N/A; Catalase (CAT) Colorimetric Assay Kit; CAT assay kit
Ordering
Specificity
96T (Can detect 40 samples without duplication)
Sequence Length
527
Samples
Serum, plasma, cells, cell culture supernatant and tissue homogenate samples
Detection Range
1.12-150 U/mL
Sensitivity
1.12 U/mL
Intra-assay Precision
The intra-assay CV is 3.9%.
Inter-assay Precision
The inter-assay CV is 7.7%.
Preparation and Storage
Store at 2-8 degree C. Valid for 12 months

Standard Curve (Sample)

product-image-AAA174668_SC15.jpg Standard Curve (Sample)
Related Product Information for CAT assay kit
Background: CAT is an enzyme in organism that can efficiently and specifically decompose hydrogen peroxide and is a binding enzyme with iron porphyrin as an auxiliary group. CAT clears hydrogen peroxide in the body and protects cells from the toxicity of H2O2. CAT can also oxidize certain cytotoxic substances, such as formaldehyde, formic acid, phenol and ethanol. According to the difference of catalytic center structure, CAT can be divided into two types, one is iron porphyrin structure, also known as iron porphyrin enzyme, the other contain manganese ion, also known as manganese catalase. CAT is common in breathing organisms. It is mainly found in chloroplasts, mitochondria, endoplasmic reticulum, liver and red blood cells of animals.

Principle of the Assay: The reaction that CAT decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
847
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
catalase
NCBI Official Synonym Full Names
catalase
NCBI Official Symbol
CAT
NCBI Protein Information
catalase
UniProt Protein Name
Catalase
UniProt Gene Name
CAT
UniProt Entry Name
CATA_HUMAN

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Product Notes

The CAT cat (Catalog #AAA174668) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Catalase (CAT), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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