Standard Curve (Sample)
Catalase (CAT) Assay Kit | CAT assay kit
Catalase (CAT) Activity Assay Kit
Synonyms
Catalase (CAT); N/A; Catalase (CAT) Activity Assay Kit; CAT assay kit
Sequence Length
527
Samples
Serum, Plasma, Animal and Plant Tissues, Cells
Application Notes
Catalase Activity Assay Kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CAT assay kit
Principle of the Assay: Catalase (CAT, EC 1.11.1.6) is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) into water and oxygen. It is widely found in aerobic cells containing cytochrome systems. Hydrogen peroxide is highly toxic to cells, and its accumulation leads to oxidation of cellular targets such as DNA, proteins and lipids, leading to mutagenesis and cell death. Protection against oxidative damage to cells is provided by using catalase to remove hydrogen peroxide (H2O2) from cells. The role of catalase in oxidative stress-related diseases has been extensively studied. Catalase also shows peroxidation activity in which low molecular weight alcohols can be used as electron donors. Fatty alcohols are specific substrates for catalase; however, other enzymes with peroxidation activity do not utilize these substrates. CheKine™ Micro Catalase (CAT) Activity Assay Kit provides a simple and easy assay for the study of catalase activity in a variety of biological samples such as serum, plasma, cells and tissue lysates or biological fluids. This assay kit utilizes the peroxidase function of catalase for measuring catalase activity, in the presence of an appropriate concentration of H2O2, catalase reacts with methanol to produce formaldehyde, which can react with chromogens. The absorbance of the product can be measured at 540 nm. The catalase activity in the sample is proportional to the OD value.
Product Categories/Family for CAT assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
catalase
NCBI Official Synonym Full Names
catalase
NCBI Official Symbol
CAT
NCBI Protein Information
catalase
UniProt Protein Name
Catalase
UniProt Gene Name
CAT
UniProt Entry Name
CATA_HUMAN
Similar Products
Product Notes
The CAT cat (Catalog #AAA31524) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Catalase Activity Assay Kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained. Researchers should empirically determine the suitability of the CAT cat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catalase (CAT), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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