Application Data
Choline/Acetylcholine Assay Kit
Choline/Acetylcholine Fluorometric Assay Kit
Synonyms
Choline/Acetylcholine; N/A; Choline/Acetylcholine Fluorometric Assay Kit; Choline/Acetylcholine assay kit
Assay Type
Quantitative
Samples
serum, plasma and animal tissue
Detection Range
0.06-10 umol/L
Sensitivity
0.06 umol/L
Detection Method
Fluorescence method
Detection Instrument
Fluorescence Microplate reader
Assay Time
30 min
Research Area
Glycolysis and Lipid Metabolism
Test Principle
Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems.Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.
Precision
Inter Assay CV: 8.2-10%
Intra Assay CV: 3.2-5%
Intra Assay CV: 3.2-5%
Preparation and Storage
This product can be stored at -20 degree Cfor 12 months with shading light.
Ship: Ice bag
Ship: Ice bag
Application Data
Application Data
Application Data
Application Data
Standard Curve (Sample)
Standard Curve (Sample)
Product Categories/Family for Choline/Acetylcholine assay kit
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Product Notes
The Choline/Acetylcholine (Catalog #AAA178636) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Choline/Acetylcholine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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