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product-image-AAA332466_SC15.jpg Standard Curve (Sample)

Diamine Oxidase (DAO) Assay Kit | DAO assay kit

Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

Synonyms
Diamine Oxidase (DAO); N/A; Diamine Oxidase (DAO) Activity Colorimetric Assay Kit; Amine oxidase; copper-containing 1; AOC1; Histaminase; DAO assay kit
Ordering
Samples
Serum, Plasma, Animal and Plant Tissues, Cells, Cell Supernatant, Bacteria, Urine
Preparation and Storage
Store at 4 degree C and keep from light immediately upon receipt. Kit has a storage time of est. 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.

Standard Curve (Sample)

product-image-AAA332466_SC15.jpg Standard Curve (Sample)
Related Product Information for DAO assay kit
Principle of the Assay: DAO (EC1.4.3.6) is widely found in animals (intestinal mucosa, lung, liver, kidney, etc.), plants and microorganisms. It catalyzes the oxidation of diamines to aldehydes. Its activity is closely related to nucleic acid and protein synthesis, and can reflect the integrity and injury degree of intestinal mechanical barrier. CheKine™ Micro Diamine Oxidase (DAO) Activity Assay Kit provides a convenient tool for detection of DAO Activity. The principle is that DAO catalyzes 1,5-pentanediamine to produce aldehyde and hydrogen peroxide. The oxidation of o-Dianisidine(3,3?-Dimethoxybenzidine) with hydrogen peroxide to produce colored substances was catalyzed by exogenous Horseradish peroxidase, the colored substances have a maximum absorption peak detected at about 460 nm. The enzyme activity of DAO was calculated by detecting the rate of increase in absorption at 460 nm. The kit can detect serum (plasma), animal and plant tissues, cells, bacteria, cell supernatants, urine and other samples.
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Product Notes

The DAO (Catalog #AAA332466) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Diamine Oxidase (DAO), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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