Application Data
Extracellular Acidification Rate (ECAR) Assay Kit | ECAR assay kit
Extracellular Acidification Rate (ECAR) Fluorometric Assay Kit
Synonyms
Extracellular Acidification Rate (ECAR); N/A; Extracellular Acidification Rate (ECAR) Fluorometric Assay Kit; ECAR assay kit
Asssay Type
Quantitative
Detection Type
Fluorescence method
Detection Instrument
Fluorescence Microplate reader
Research Area
Glycolysis and Lipid Metabolism
Test Principle
The extracellular acidification rate (ECAR) refers to the effect of acidic substances released by cells during metabolism on the extracellular environment, which is an important indicator to evaluate the energy metabolism and growth status of cells. During cellular metabolism, energy generation requires either aerobic or anaerobic respiration. When cells undergo anaerobic respiration, a large number of acidic substances are produced, which are pumped by the cell membrane into the extracellular environment, leading to the acidification of the extracellular environment. Therefore, ECAR is an important indicator to evaluate the acidic substances produced by anaerobic respiration during cell metabolism, and its change can reflect the degree of cell metabolism.The kit uses a specific probe (Ex/Em: 490 nm/535 nm) that changes the fluorescence value when the pH of the extracellular environment changes. The acidified extracellular environment will reduce the fluorescence value of the probe to detect the extracellular acidification rate.
Preparation and Storage
This product can be stored at -20 degree C for 12 months with shading light.
Shipping: Ice bag
Shipping: Ice bag
Application Data
Application Data
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Product Notes
The ECAR (Catalog #AAA179636) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Extracellular Acidification Rate (ECAR), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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