Glucuronidase Assay Kit
Glucuronidase Sample Prep kit (to accompany EIA kits)
Synonyms
Glucuronidase; N/A; Glucuronidase Sample Prep kit (to accompany EIA kits); Glucuronidase assay kit
Experimental Procedure
1. For every 100 uL of urine to be assayed, add 5 uL of Beta-Glucuronidase. Seal container and mix solution by inversion.
2. Incubate the mixture at 37°C for 2 hours.
3. The sample is now ready for dilution and assay.
2. Incubate the mixture at 37°C for 2 hours.
3. The sample is now ready for dilution and assay.
Preparation and Storage
This product is stable for at least 6 months at 4°C. For long term storage, freeze at -20°C or -80°C.
Related Product Information for Glucuronidase assay kit
Isoprostanes are primarily generated by free radical mechanisms making them excellent biomarkers for systemic oxidant stress. It is now known that isoprostanes are extensively metabolized in humans. We have found that an average of 50% of the isoprostane excreted in human urine is conjugated to glucuronic acid. The extent of glucuronidation among individuals ranges significantly from 28% to 80%. In light of this information it is strongly recommended that specimens be pretreated with Beta- glucuronidase prior to isoprostane analysis to provide a more accurate assessment of oxidative stress. This kit provides sufficient materials and methods for the treatment of 40 samples allowing the user to differentiate the inter-individual differences in glucuronidation and measure the total systemic isoprostane output.
Similar Products
Product Notes
The Glucuronidase (Catalog #AAA71817) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glucuronidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.