Standard Curve (Sample)
Glutamine Assay Kit
Glutamine Assay Kit (Fluorometric)
Synonyms
Glutamine; N/A; Glutamine Assay Kit (Fluorometric); Glutamine assay kit
Detection Method
Fluorometric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Glutamine assay kit
Background: Glutamine is a conditionally essential amino acid (needed from the diet under stress conditions) that is used in protein biosynthesis. The glutamine side chain is almost identical to glutamic acid, except the carboxylic acid group on the side chain is replaced by an amide group. Glutamine is the most abundant free amino acid in human blood. Many food sources are rich in glutamine including proteinrich foods such as dairy products, eggs, fish, chicken, beef, as well as vegetables like beans, beets, cabbage, spinach, and carrots. In addition to protein synthesis/function, glutamine plays a role in acidbase balance in the kidney by producing ammonium, as a source of cellular energy next to glucose, and as a nitrogen donator for many anabolic processes such as the synthesis of purine. Cancer cells rely on glutamine as a nitrogen and carbon source. The importance of glutamine in certain tumors has been demonstrated. For example, blocking glutamine metabolism was shown to inhibit growth of several tumors such as breast, liver, kidney and T-cell lymphoblastic leukemia. The Glutamine Assay Kit is a simple assay for measuring glutamine levels in biological samples without any need for pretreatment. The kit has a detection sensitivity limit of 1.56 uM glutamine. Each kit provides sufficient reagents to perform up to 200 assays*, including standard curve and unknown samples.
Principle of the Assay: The Glutamine Assay Kit is a sensitive quantitative fluorometric assay for glutamate. Glutaminase converts glutamine and H2O to glutamate and NH3. Glutamate oxidase converts glutamate to alpha- ketoglutarate and NH3 as well as H2O2. L-alanine and glutamate-pyruvate transaminase are also added to the reaction in order to regenerate glutamate and H2O2. As a result, multiple rounds of the reaction occur which results in significant amplification of H2O2 production. In the presence of HRP, the Fluorometric Probe reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm (Figure 1). Fluorescence values are proportional to the glutamine levels within the samples. The glutamine content in unknown samples is determined by comparison with a standard curve.
Principle of the Assay: The Glutamine Assay Kit is a sensitive quantitative fluorometric assay for glutamate. Glutaminase converts glutamine and H2O to glutamate and NH3. Glutamate oxidase converts glutamate to alpha- ketoglutarate and NH3 as well as H2O2. L-alanine and glutamate-pyruvate transaminase are also added to the reaction in order to regenerate glutamate and H2O2. As a result, multiple rounds of the reaction occur which results in significant amplification of H2O2 production. In the presence of HRP, the Fluorometric Probe reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm (Figure 1). Fluorescence values are proportional to the glutamine levels within the samples. The glutamine content in unknown samples is determined by comparison with a standard curve.
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Product Notes
The Glutamine (Catalog #AAA44696) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glutamine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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