Standard Curve (Sample)
Glutathione Reductase Assay Kit | GSR assay kit
Glutathione Reductase Assay Kit
Gene Names
GSR; HEL-75
Synonyms
Glutathione Reductase; N/A; Glutathione Reductase Assay Kit; Spectrophotometric Assay for Glutathione Reductase; GSR assay kit
Sequence Length
230
Preparation and Storage
4 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GSR assay kit
Principle of the Assay: This assay is based on the oxidation of NADPH to NADP+ catalyzed by a limiting concentration of glutathione reductase. One GR activity unit is defined as the amount of enzyme catalyzing the reduction of one micromole of GSSG per minute at pH 7.6 and 25 degree C. As shown in the above reaction, one molecule of NADPH is consumed for each molecule of GSSG reduced. Therefore, the reduction of GSSG is determined indirectly by the measurement of the consumption of NADPH, as demonstrated by a decrease in absorbance at 340 nm (A340) as a function of time.
References
1. Dolphin D, Poulson R, Avramovic O, Eds. (1989) Glutathione: Chemical, Biochemical and Metabolic Aspects, Vols. A and B, J. Wiley and Sons.
2. Bashir A, Perham RN, Scrutton NS, Berry A (1995) "Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase" Biochem.J. 312, 527-533.
3. Massey V, Williams CH (1965) "On the mechanism of yeast glutathione reductase" J.Biol.Chem. 240 (11), 4470-4480.
4. Worthington DJ, Rosemeyer MA (1976) "Glutathione reductase from human erythrocytes. Catalytic properties and aggregation" Eur.J.Biochem. 67, 231-238.
5. García-Alfonso C, Martìnez-Galisteo, Llobell A, Bárcena JA, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (1), 61-68.
6. Carlberg I, Mannervik B (1985) "Glutathione reductase" Meth.Enzymol. 113, 485-490.
7. Peindo J, García-Alfonso C, Martínez-Galisteo E, Llobell A, López-Barea J (1991) "Metals are directly involved in the redox interconversion of Saccharomyces cervisiae glutathione reductase" Molec.Cell.Biol. 101, 175-187.
8. García-Alfonso C, Martínez-Galisteo E, Llobell A, Bácena J, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (4), 513-520.
9. Smith I, Vierheller T, Thorne C (1988) "Assay of glutathione reductase in crude tissue homogenates using 5,5'- dithiobis(2-nitrobenzoic acid)" Anal.Biochem. 75, 408-413.
10. Beutler, E (1969) "Effect of flavin compounds on the glutathione reductase activity: in vitro and in vivo studies" J.Clin.Invest. 48, 1957-1966.
2. Bashir A, Perham RN, Scrutton NS, Berry A (1995) "Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase" Biochem.J. 312, 527-533.
3. Massey V, Williams CH (1965) "On the mechanism of yeast glutathione reductase" J.Biol.Chem. 240 (11), 4470-4480.
4. Worthington DJ, Rosemeyer MA (1976) "Glutathione reductase from human erythrocytes. Catalytic properties and aggregation" Eur.J.Biochem. 67, 231-238.
5. García-Alfonso C, Martìnez-Galisteo, Llobell A, Bárcena JA, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (1), 61-68.
6. Carlberg I, Mannervik B (1985) "Glutathione reductase" Meth.Enzymol. 113, 485-490.
7. Peindo J, García-Alfonso C, Martínez-Galisteo E, Llobell A, López-Barea J (1991) "Metals are directly involved in the redox interconversion of Saccharomyces cervisiae glutathione reductase" Molec.Cell.Biol. 101, 175-187.
8. García-Alfonso C, Martínez-Galisteo E, Llobell A, Bácena J, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (4), 513-520.
9. Smith I, Vierheller T, Thorne C (1988) "Assay of glutathione reductase in crude tissue homogenates using 5,5'- dithiobis(2-nitrobenzoic acid)" Anal.Biochem. 75, 408-413.
10. Beutler, E (1969) "Effect of flavin compounds on the glutathione reductase activity: in vitro and in vivo studies" J.Clin.Invest. 48, 1957-1966.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,081 Da
NCBI Official Full Name
glutathione reductase, partial
NCBI Official Synonym Full Names
glutathione reductase
NCBI Official Symbol
GSR
NCBI Official Synonym Symbols
HEL-75
NCBI Protein Information
glutathione reductase, mitochondrial; GR; GRase; epididymis luminal protein 75
UniProt Protein Name
Glutathione reductase
UniProt Entry Name
Q03504_HUMAN
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Product Notes
The GSR (Catalog #AAA71812) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glutathione Reductase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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