Application Data
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit | GAPDH assay kit
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit
Synonyms
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH); N/A; Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit; GAPDH assay kit
Asssay Type
Enzyme Activity
Samples
tissue, cell
Detection Range
0.49-50.00 U/L
Sensitivity
0.49 U/L
Detection Type
Colorimetric method
Detection Instrument
Microplate reader
Precision
Inter CV: 3.2-8.6%
Intra CV: 3.2-5%
Intra CV: 3.2-5%
Research Area
Glycolysis and Lipid Metabolism
Test Principle
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body.This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.
Preparation and Storage
This product can be stored at -20 degree C for 12 months with shading light.
Shipping: Ice bag
Shipping: Ice bag
Application Data
Application Data
Application Data
Application Data
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Product Notes
The GAPDH (Catalog #AAA179646) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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