Glycolate Oxidase Assay Kit
Glycolate Oxidase Activity Assay Kit
Synonyms
Glycolate Oxidase; N/A; Glycolate Oxidase Activity Assay Kit; Glycolate Oxidase assay kit
Samples
Plant tissue samples
Detection Range
0.3-350U/mL
Sensitivity
0.3U/mL
Precision
Average inter-assay CV: 8.2%
Average intra-assay CV: 1.1%
Average intra-assay CV: 1.1%
Test Principle
Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
Preparation and Storage
Store at 2-8 degree C. Valid for 12 months
Related Product Information for Glycolate Oxidase assay kit
Background: Glycolate oxidase, a non-mitochondrial oxidase, is found in plant cell peroxidasomes and can catalyze the oxidation of glycolic acid to glyoxylic acid and release hydrogen peroxide. Glyoxylic oxidase is one of the key enzymes in plant photorespiration, and its activity can be used as an important index to measure the intensity of plant photorespiration, which changes with plant development, mineral nutrition and disease susceptibility.
Principle of the Assay: Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
Intended Uses: This kit can be used to measure glycolate oxidase activity in plant tissue samples.
Principle of the Assay: Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
Intended Uses: This kit can be used to measure glycolate oxidase activity in plant tissue samples.
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Product Notes
The Glycolate Oxidase (Catalog #AAA177835) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glycolate Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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