Standard Curve (Sample)
Guanosine Assay Kit
Guanosine Assay Kit
Synonyms
Guanosine; N/A; Guanosine Assay Kit; Guanosine assay kit
Preparation and Storage
Upon receipt, store the 10X Assay Buffer at room temperature and store the rest of the kit at -20 degree C. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Guanosine assay kit
Principle of the Assay: The Guanosine Assay Kit measures total Guanosine within biological samples. Guanosine is converted to Guanine by PNP. Guanine is converted into xanthine by guanine deaminase. Then xanthine is converted to uric acid and hydrogen peroxide by xanthine oxidase. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of Guanosine standard within the 96-well microtiter plate format. Samples and standards are incubated for 15 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Guanosine is a purine nucleoside made up of a guanine moiety attached to a ribose ring through a beta-N9- glycosidic bond. Guanosine can be converted to guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP) by the process of phosphoryation. These phosphorylated forms of guanosine play key roles in the processes of nucleic acid and protein synthesis, photosynthesis, muscle contraction, and intracellular signal transduction (specifically with cGMP). Guanosine is required in mRNA for an RNA splicing reaction. When a "self-splicing" intron excises itself from the mRNA message (requiring a 5' guanosine) cutting at both ends, re-ligating, and leaving just the exons on either side to be translated into a functional protein. The antiviral drug acyclovir, which is used in the treatment of herpes, and the anti-HIV drug abacavir are both structurally similar to guanosine. Guanosine was also used to make regadenoson, an A2A adenosine receptor agonist that is a coronary vasodilator and is often used in pharmacologic stress testing. Guanosine prevents Quinolinic Acid (QA) induced seizures about as well as the NMDA receptor antagonist, MK-801. Besides acting as an NMDA receptor agonist, QA decreases glutamate transport/uptake. It has been shown that Guanosine counteracts the QA-induced decrease in glutamate uptake as well as increases in glutamate release from the synapse. The Guanosine Assay Kit is a simple fluorometric assay that measures the amount of total Guanosine present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, Guanosine standards, and unknown samples. Sample Guanosine concentrations are determined by comparison with a known Guanosine standard. The kit has a detection sensitivity limit of 1.6 uM Guanosine.
Background/Introduction: Guanosine is a purine nucleoside made up of a guanine moiety attached to a ribose ring through a beta-N9- glycosidic bond. Guanosine can be converted to guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP) by the process of phosphoryation. These phosphorylated forms of guanosine play key roles in the processes of nucleic acid and protein synthesis, photosynthesis, muscle contraction, and intracellular signal transduction (specifically with cGMP). Guanosine is required in mRNA for an RNA splicing reaction. When a "self-splicing" intron excises itself from the mRNA message (requiring a 5' guanosine) cutting at both ends, re-ligating, and leaving just the exons on either side to be translated into a functional protein. The antiviral drug acyclovir, which is used in the treatment of herpes, and the anti-HIV drug abacavir are both structurally similar to guanosine. Guanosine was also used to make regadenoson, an A2A adenosine receptor agonist that is a coronary vasodilator and is often used in pharmacologic stress testing. Guanosine prevents Quinolinic Acid (QA) induced seizures about as well as the NMDA receptor antagonist, MK-801. Besides acting as an NMDA receptor agonist, QA decreases glutamate transport/uptake. It has been shown that Guanosine counteracts the QA-induced decrease in glutamate uptake as well as increases in glutamate release from the synapse. The Guanosine Assay Kit is a simple fluorometric assay that measures the amount of total Guanosine present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, Guanosine standards, and unknown samples. Sample Guanosine concentrations are determined by comparison with a known Guanosine standard. The kit has a detection sensitivity limit of 1.6 uM Guanosine.
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Product Notes
The Guanosine (Catalog #AAA44682) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Guanosine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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