Samples
Serum, plasma, cell culture supernatants, tissue/cell lysates and other biological fluids
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Related Product Information for H2O2 assay kit
Principle of the Assay: Abbkine Hydrogen Peroxide Assay Kit provides a simple and easy colorimetric assay for measuring hydrogen peroxide in serum, plasma, cell culture supernatants, tissue/cell lysates and other biological fluids. In the assay, H2O2 oxidize ferrous to ferric ion, and xylenol orange binds ferric ion with high selectivity to form a colored (purple) product that can be measured by colorimetric method at OD 580 nm. The Hydrogen Peroxide present in the sample is proportional to the signal obtained.!!Background/Introduction: Hydrogen Peroxide (H2O2), a reactive oxygen metabolic by-product, which serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neurodegenerative diseases, Down's syndrome and immune system diseases.
Product Categories/Family for H2O2 assay kit
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Product Notes
The H2O2 (Catalog #AAA332450) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hydrogen Peroxide (H2O2), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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