Hydroxyl Free Radical Scavenging Capacity Assay Kit
Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit
Synonyms
Hydroxyl Free Radical Scavenging Capacity; N/A; Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit; Hydroxyl Free Radical Scavenging Capacity assay kit
Samples
Animal and Plant Tissues, Serum, Plasma, Cells, Bacteria, Cell Supernatant, Juice, Honey, and Urine
Usage Notes
Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.
If the OD value exceeds 1.3, the sample needs to be diluted.
The sample is hemolyzed or has a dark color and needs to be compared with itself. The specific method is to add 200 uL of the diluted sample and 30 uL of Extraction Buffer (1x) to UV microplate, incubate at 37 degree C for 5 min, record the absorbance at 340nm, and subtract the OD value of the self-control from the measurement well during calculation.
Serum, plasma and tissue samples are recommended to be diluted 5 times with Extraction Buffer (1x) for testing.
The Reagent I and Reagent II are stored in sealed condition.
Avoid bubbles while mixing or redissolving components.
Change pipette tips frequently to avoid cross contamination between components.
Ensure that all components and equipment are at the proper temperature before starting the experiment.
In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.
If the OD value exceeds 1.3, the sample needs to be diluted.
The sample is hemolyzed or has a dark color and needs to be compared with itself. The specific method is to add 200 uL of the diluted sample and 30 uL of Extraction Buffer (1x) to UV microplate, incubate at 37 degree C for 5 min, record the absorbance at 340nm, and subtract the OD value of the self-control from the measurement well during calculation.
Serum, plasma and tissue samples are recommended to be diluted 5 times with Extraction Buffer (1x) for testing.
The Reagent I and Reagent II are stored in sealed condition.
Preparation and Storage
Store at 4 degree C. Kit has a storage time of 12 months from receipt. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for Hydroxyl Free Radical Scavenging Capacity assay kit
Principle of the Assay: The hydroxyl radical·OH, is the neutral form of the hydroxide ion (OH -), highly reactive (easily becoming hydroxyl groups).Hydroxyl free radicals act on biological molecules such as proteins, nucleic acids, and lipids in the body, causing damage to cell structure and function, which in turn leads to metabolic disorders in the body and causes diseases. Hydroxyl free radical scavenging capacity is one of the important indicators of the antioxidant capacity of samples, and it has been widely used in theresearch of antioxidant health products and drugs. CheKine™ Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit isspecially developed for the detection of hydroxyl free radical scavenging capacity in various sample. The operation is simple andconvenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through theFenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce2,3-dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavengehydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflect the hydroxyl free radical scavenging capacity of the sample.
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Product Notes
The Hydroxyl Free Radical Scavenging Capacity (Catalog #AAA332508) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hydroxyl Free Radical Scavenging Capacity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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