Standard Curve (Sample)
IGG1 assay kit
IGG1 Immunoassay
Synonyms
IGG1; N/A; IGG1 Immunoassay; IGG1 assay kit
Assay Type
Sandwich
Preparation and Storage
Store at 4 degree C
Shelf life: 6 Months
Shelf life: 6 Months
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IGG1 assay kit
Background: Recombinant human monoclonal antibodies have become a major focus of new drug discovery in the Pharmaceutical and Biotech industries, with hundreds of new human monoclonal antibodies currently in preclinical development. Fully human recombinant antibodies have gradually replaced humanized mouse or rat antibodies, with IgG1 and IgG4 as the preferred engineered isotypes2. As part of the developmental process, human therapeutic antibody drug candidate molecules must be tested in non-human primate species to assess their pharmacokinetic profile and potential toxicity3. Primate and human antibodies are highly homologous, making it difficult to distinguish the human antibody drug candidate from the endogenous primate antibodies in a serum or plasma sample by traditional human IgG-specific immunoassays. Therefore, we developed two highly sensitive ELISAs that distinguish human IgG1 or human IgG4 from rhesus or cynomolgus monkey immunoglobulins. This Recombinant (Therapeutic) Human IgG1 ELISA can be used to accurately quantify human IgG1 in the serum or plasma of rhesus or cynomolgus monkeys without cross reacting with antibodies of those species. This same ELISA can also be used to quantify human IgG1 in the plasma of other preclinical test species (mouse, rat, rabbit, guinea pig, etc.) if desired. This assay is supplied with a recombinant human IgG1 kappa allotype G1m17.1 standard. The customer may wish to substitute their own specific drug candidate IgG1 for this standard in the assay.
Principle of the Assay: This is a sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-Human IgG Antibody and blocked, ready for the addition of samples and standards. The assay should take approximately 3 hours to run, plus any required sample preparation time.
Principle of the Assay: This is a sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-Human IgG Antibody and blocked, ready for the addition of samples and standards. The assay should take approximately 3 hours to run, plus any required sample preparation time.
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Product Notes
The IGG1 (Catalog #AAA71853) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IGG1, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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