Standard Curve (Sample)
Lipid Peroxidation (MDA) Assay Kit
Lipid Peroxidation (MDA) Assay kit
Synonyms
Lipid Peroxidation (MDA); N/A; Lipid Peroxidation (MDA) Assay kit; Lipid Peroxidation (MDA) assay kit
Samples
Serum, Plasma, Urine, Animal and Plant Tissues, Cells, Bacteria
Application Notes
Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct, which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).
Preparation and Storage
Store at -20 degree C immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Lipid Peroxidation (MDA) assay kit
Principle of the Assay: Oxygen free radicals react on lipid unsaturated fatty acids to produce lipid peroxidation. The latter gradually breaks down into a series of complex compounds, including malondialdehyde (MDA). Lipid oxidation levels can be measured by detecting MDA levels. Lipid peroxidation may contribute to many diseases, including atherosclerosis, diabetes, and Alzheimer's disease. CheKine™ Micro Lipid Peroxidation (MDA) Assay kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. Malondialdehyde (MDA) can be condensed with thiobarbituric acid (TBA) under acidic and high temperature conditions to produce the brown-red trimethyltran (3,5, 5-trimethyloxazol-2, 4-dione) with a maximum absorption wavelength of 532 nm. Meanwhile, the absorbance at 600 nm was measured to eliminate the interference of sucrose, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm.
Product Categories/Family for Lipid Peroxidation (MDA) assay kit
Similar Products
Product Notes
The Lipid Peroxidation (MDA) (Catalog #AAA31525) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct, which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose). . Researchers should empirically determine the suitability of the Lipid Peroxidation (MDA) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipid Peroxidation (MDA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.