Standard Curve (Sample)
Lipoprotein Lipase (LPL) Activity Assay Kit
Lipoprotein Lipase (LPL) Activity Assay Kit (Fluorometric)
Synonyms
Lipoprotein Lipase (LPL) Activity; N/A; Lipoprotein Lipase (LPL) Activity Assay Kit (Fluorometric); Lipoprotein Lipase (LPL) Activity assay kit
Preparation and Storage
Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The LPL Fluorometric Substrate is light sensitive and should be maintained in amber tubes.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Lipoprotein Lipase (LPL) Activity assay kit
Background/Introduction: Triglycerides (TAG) are a type of lipid in the blood serving as an energy source and playing a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lipases hydrolyse the triglyceride ester bond, yielding glycerol and free fatty acids in a process called lipolysis. Enterocytes then absorb and repackage the fragments with cholesterol to form chylomicrons, a major lipoprotein transport particle. In the liver, hepatic lipases also break down triglycerides to assemble another lipoprotein particle (VLDL) from triglycerides, cholesterol, and apolipoproteins. Plasma triglyceride levels are regulated by the assembly and degradation of VLDL and chylomicron particles. Lipoprotein lipase (LPL) is the key plasma lipase responsible for hydrolysis of the triglyceride core in these particles. Lipoprotein Lipase Activity Assay Kit utilizes a fluorogenic triglyceride analog as a lipase substrate. When uncleaved, the substrate remains in a non-fluorescent, quenched state; however, upon hydrolysis by LPL at the sn-1 position, a fluorescent product is produced which can be measured in a fluorescence microplate reader (Ex. 480-485 nm/Em. 515-525 nm with 495 nm cutoff) (See Assay Principle). The Lipoprotein Lipase (LPL) Activity Assay Kit is a simple, fluorometric assay that quantitatively measures LPL activity in plasma, serum, and lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, LPL standards, and unknown samples. The kit contains a LPL Standard and has a detection sensitivity limit of ~1 mUnits/mL (see Kit Components for unit definition). Besides LPL, this assay can also be used to detect endothelial and hepatic lipase activity.
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Product Notes
The Lipoprotein Lipase (LPL) Activity (Catalog #AAA44526) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lipoprotein Lipase (LPL) Activity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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