Standard Curve (Sample)
Low-density lipoprotein cholesterol(LDL) Assay Kit | LDL assay kit
Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)
Synonyms
Low-density lipoprotein cholesterol(LDL); N/A; Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer); LDL assay kit
Specificity
96T (92 samples)
Samples
Serum, plasma, cells, culture supernatant and tissue samples
Detection Range
0.04-12 mmol/L
Sensitivity
0.04 mmol/L
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
Preparation and Storage
Store at 2-8 degree C. Valid for 12 months
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LDL assay kit
Principle of the Assay: Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. The coloured substance have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the LDL-C content in the sample can be calculated.
Intended Uses: This kit can be used for detection of low-density lipoprotein cholesterol (LDL-C) content in serum, plasma, cells, culture supernatant and tissue samples.
Intended Uses: This kit can be used for detection of low-density lipoprotein cholesterol (LDL-C) content in serum, plasma, cells, culture supernatant and tissue samples.
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Product Notes
The LDL (Catalog #AAA172734) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Low-density lipoprotein cholesterol(LDL), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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